Description

Cultrex®BasementMembraneExtract(BME)isasolubleformofbasementmembranepurifiedfromEngelbreth-Holm-Swarm(EHS)tumor.Thisextractprovidesanaturalextracellularmatrixhydrogelthatpolymerizesat37°Ctoformareconstitutedbasementmembrane.Basementmembranesarecontinuoussheetsofspecializedextracellularmatrixthatformaninterfacebetweenendothelial,epithelial,muscle,orneuronalcellsandtheiradjacentstromaandthatplayanessentialroleintissueorganizationbyinfluencingcelladhesion,migration,proliferation,anddifferentiation.ThemajorcomponentsofBMEincludelaminin,collagenIV,entactin,andheparinsulfateproteoglycans.

Cultrex®BMEisaBasementMembraneMatrix thathasbeendeveloped,producedandqualifiedforgeneralcellcultureapplications.

Qualitycontrolspecifications:

• Mousecoloniesareroutinelyscreenedforpathogensusing mouseantibodyproduction(MAP)testing.
• Engelbreth-Holm-Swarm(EHS)tumorisLDEV-free.
• PathClear®–testednegativebyPCRfor17bacterialandvirusstrainstypicallyincludedinmouseantibodyproduction(MAP)testing,plus13additionalmurineinfectiousagentsincludingLDEV,foratotalof31organismsandviruses.
• Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
• Endotoxinconcentration≤8EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
• Proteinconcentrationof12–18mg/ml.
• NomycoplasmacontaminationdetectedbyPCR.
• ProvidedinDulbecco’sModifiedEagle’smedium(DMEM)withoutphenolred,with10µg/mlgentamicinsulfate.
• GelstABIlitytestedforaperiodof14daysat37°C.
• BIOLOGicalactivitydeterminedforeachlot:
  • Tubeformationassay–BMEpromotesformationofcapillary-likestructuresbyhuman(HBMVEC;HUVEC)ormouse(SVEC4-10)endothelialcells.

“Dickkopf-3regulatesprostateepithelialcellacinarmorphogenesisandprostatecancercellinvasionbylimitingTGF-β-dependentactivationofmatrixmetalloproteases”
“DianaRomero,ZainabAl-Shareef,IrantzuGorroño-Etxebarria,StephanieAtkins,FrancesTurrell,Jyoti
Chhetri,NoraBengoa-Vergniory,ChristophZenzmaier,PeterBerger,JonathanWaxman,andRobertKypta”
Carcinogenesis,Jan2016;37:18–29
http://carcin.oxfordjournals.org/cgi/content/abstract/37/1/18

Real-timemonitoringofNKCC2endocytosisbytotalinternalreflectionfluorescence(TIRF)microscopy
AnkitaBachhawatJaykumar,PauloS.Caceres,IbrahimSablaban,BakhosA.Tannous,andPabloA.Ortiz
AmJPhysiolRenalPhysiol,Jan2016;310:F183–F191
http://ajprenal.physiology.org/cgi/content/abstract/310/2/F183

Cabozantinib(XL184)InhibitsGrowthandInvasionofPreclinicalTNBCModels
“MansourehSameni,ElizabethA.Tovar,CurtJ.Essenburg,AnitaChalasani,ErikS.Linklater,Andrew
Borgman,DavidM.Cherba,ArulselviAnbalagan,MaryE.Winn,CarrieR.Graveel,andBonnieF.Sloane
Clin.CancerRes.,Feb2016;22:923–934”
http://clincancerres.aacrjournals.org/cgi/content/abstract/22/4/923

3-DimensionalPatient-DerivedLungCancerAssaysRevealResistancetoStandards-of-CarePromotedbyStromalCellsbutSensitivitytoHistoneDeacetylaseInhibitors
DavidOnion,RichardH.Argent,AlexanderM.Reece-Smith,MadeleineL.Craze,RobertG.Pineda,PhilipA.Clarke,HariL.Ratan,SimonL.Parsons,DileepN.Lobo,JohnP.Duffy,JohnC.Atherton,AndrewJ.McKenzie,RajendraKumari,PeterKing,BrettM.Hall,andAnnaM.Grabowska
Mol.CancerTher.,Apr2016;15:753–763
http://mct.aacrjournals.org/cgi/content/abstract/15/4/753

SuppressionofER{beta}signalingviaER{beta}knockoutorantagoNISTprotectsagainstbladdercancerdevelopment
Hsu,I.,Chuang,K.L.,Slavin,S.,Da,J.,Lim,W.X.,Pang,S.T.,O’Brien,J.H.andYeh,S.
Carcinogenesis,Mar2014;35:651–661
http://carcin.oxfordjournals.org/cgi/content/abstract/35/3/651

Theinterplaybetweenhypoxia,endothelialandmelanomacellsregulatesvascularizationandcellmotilitythroughendothelin-1andvascularendothelialgrowthfactor,
FrancescaSpinella,ValentinaCaprara,RobertaCianfrocca,LauraRosanò,ValerianaDiCastro,EmirenaGarrafa,PierGiorgioNatali,andAnnaBagnato
Carcinogenesis,Apr2014;35:840–848
http://carcin.oxfordjournals.org/cgi/content/abstract/35/4/840

InhibitionofTumorGrowthandAngiogenesisbySP-2,anAnti-Lectin,Galactoside-BindingSoluble3BindingProteinLGALS3BPAntibody
SaraTraini,EnzaPiccolo,NicolaTinari,CosmoRossi,RossanaLaSorda,FrancescaSpinella,AnnaBagnato,RossanoLattanzio,MauriziaD’Egidio,AnnalisaDiRisio,FedericaTomao,AntoninoGrassadonia,MauroPiantelli,ClaraNatoli,andStefanoIacobelli
Mol.CancerTher.,Apr2014;13:916–925.
http://mct.aacrjournals.org/cgi/content/abstract/13/4/916

SimianImmunodeficiencyVirusandHumanImmunodeficiencyVirusType1MatrixProteinsSpecifyDifferentCapabilitiesToModulateBCellGrowth
Caccuri,F.,Giagulli,C.,Reichelt,J.,Martorelli,D.,Marsico,S.,Bugatti,A.,Barone,I.,Rusnati,M.,Guzman,CA.,Dolcetti,R.andCaruso,A.
J.Virol.,May2014;88:5706–5717.
http://jvi.asm.org/cgi/content/abstract/88/10/5706

TheNuclearReceptorPeroxisomeProliferator-activatedReceptor-β/(PPARβ/)PromotesOncogene-inducedCellularSenescencethroughRepressionofEndoplasmicReticulumStress
BokaiZhu,ChristinaH.Ferry,LaurenK.Markell,NicholasBlazanin,AdamB.Glick,FrankJ.Gonzalez,andJeffreyM.Peters
J.Biol.Chem.,Jul2014;289:20102–20119.
http://www.jbc.org/cgi/content/abstract/289/29/20102

PI3K/mTORDualInhibitorVS-5584PreferentiallyTargetsCancerStemCells
VihrenN.Kolev,QuentinG.Wright,ChristianM.Vidal,JenniferE.Ring,IrinaM.Shapiro,JillRicono,DavidT.Weaver,MaheshV.Padval,JonathanA.Pachter,andQunliXu
CancerRes.,Jan2015;75:446–455.
http://cancerres.aacrjournals.org/cgi/content/abstract/75/2/446

TheMEF2–HDACaxiscontrolsproliferationofmammaryepithelialcellsandaciniformationinvitro
AndreaClocchiatti,ErosDiGiorgio,GiuliaViviani,CharlesStreuli,AndreaSgorbissa,RaffaellaPicco,ValentinaCutano,andClaudioBrancolini
J.CellSci.,Nov2015;128:3961–3976.
http://jcs.biologists.org/cgi/content/abstract/128/21/3961

Dickkopf-3regulatesprostateepithelialcellacinarmorphogenesisandprostatecancercellinvasionbylimitingTGF-β-dependentactivationofmatrixmetalloproteases
DianaRomero,ZainabAl-Shareef,IrantzuGorroño-Etxebarria,StephanieAtkins,FrancesTurrell,JyotiChhetri,NoraBengoa-Vergniory,ChristophZenzmaier,PeterBerger,JonathanWaxman,andRobertKypta
Carcinogenesis,Nov2015;10.1093/carcin/bgv153
http://carcin.oxfordjournals.org/cgi/content/abstract/bgv153v2

MELK-T1,asmall-moleculeinhibitorofproteinkinaseMELK,decreasesDNA-damagetoleranceinproliferatingcancercells
LijsBeke,CenkKig,JoannesT.M.Linders,ShannahBoens,AnBoeckx,ErikavanHeerde,MarcParade,AnDeBondt,IlseVandenWyngaert,TarigBashir,SouichiOgata,LievenMeerpoel,AleydeVanEynde,ChristopherN.Johnson,MoniqueBeullens,DirkBrehmer,andMathieuBollen
Biosci.Rep.,Nov2015;35:e00267.
http://www.bioscirep.org/cgi/content/abstract/35/6/e00267

“CombinedEffectsofSuberoylanilideHydroxamicAcidandCisplatinonRADIationSensitivityandCancerCellInvasioninNon–SmallCellLungCancer”
JianguoFeng,ShirongZhang,KanWu,BingWang,JeffreyY.C.Wong,HongJiang,RujunXu,LishaYing,HaixiuHuang,XiaoliangZheng,XufengChen,andShenglinMa
Mol.CancerTher.,May2016;15:842–853.
http://mct.aacrjournals.org/cgi/content/abstract/15/5/842

Trevigen提供多种产品来测量氧化应激和DNA损伤。我们的彗星实验®产品线是为彗星试验，其中包括试剂，试剂盒，设备和分析软件的唯一完整和优化的商用平台。我们的TACS ®（Trevigen公司凋亡的细胞系统）产品已被广泛使用，并列举多年来，研究人员对他们的无与伦比的品质认可。