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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Trevigen/Cultrex® 3D Culture Matrix Rat Collagen I/3447-020-01/20 ml
产品编号:3447-020-01
市  场 价:¥3300.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$165.00
品      牌: Trevigen
公      司:Trevigen, Inc
公司分类:
Trevigen/Cultrex® 3D Culture Matrix Rat Collagen I/3447-020-01/20 ml
商品介绍

Description

Cultrex®3DCultureMatrix®CollagenI ispurifiedfromrattailtendons and isprovidedatahighconcentrationthatiscapableofpolymerizingtoforma hydrogel.CollagenIisthemajorstructuralcomponentofextracellularmatrices(ECM)foundinconnectivetissueandinternalorgans,butismostprevalentinthedermis,tendons,andbone.Itisa300kDamoleculecomposedoftwoalpha1(I)chainsandonealpha2(I)chainthatspontaneouslyformsatriplehelixscaffoldataneutralpHand37̊C.Thisphenomenoncanbeexploitedtopromotecellattachment,growth,differentiation,migration,andtissuemorphogenesisduringdevelopment.ToprovidethemoststandardizedCollagenIforusein3-Dcultures,aspecialprocessisemployedtoprovidematerialatastandardconcentrationofapproximately5mg/mL.Thismaterialisthenincorporatedina3-Dculturetovalidateefficacy.

Cultrex®3DCultureMatrix®CollagenI isapurifiedstromalECMprotein thathasbeendeveloped,producedandqualifiedspecificallyforusein3Dculturestudies.

Qualitycontrolspecifications:

  • Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
  • Endotoxinconcentration≤20EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
  • NomycoplasmacontaminationdetectedbyPCR.
  • Proteinconcentrationof5mg/ml.
  • Providedin 20mMAceticAcid,pH3.4–3.6.
  • BIOLOGicalactivitydeterminedforeachlot:
    • 3-DCulture:CollagenIformsagelwhendilutedto0.4mg/mlatneutralpHandpromotesattachmentandgrowthofmurineendothelialSVEC4-10cells.
    • CellAttachment:TestedfortheABIlitytopromotecellattachmentandspreADIngofHT1080humanfibrosarcomacells.

“OrganotypicCultureofUntransformedandTumOrigenicPrimaryMammaryEpithelialCells”
MartinJechlinger
ColdSpringHarbProtoc,May2015;2015:pdb.prot078295
http://cshprotocols.cshlp.org/cgi/content/abstract/2015/5/pdb.prot078295

品牌介绍

Trevigen提供多种产品来测量氧化应激和DNA损伤。我们的彗星实验®产品线是为彗星试验,其中包括试剂,试剂盒,设备和分析软件的唯一完整和优化的商用平台。我们的TACS ®(Trevigen公司凋亡的细胞系统)产品已被广泛使用,并列举多年来,研究人员对他们的无与伦比的品质认可。

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