Description
Cultrex®3DCultureMatrix®CollagenI ispurifiedfromrattailtendons and isprovidedatahighconcentrationthatiscapableofpolymerizingtoforma hydrogel.CollagenIisthemajorstructuralcomponentofextracellularmatrices(ECM)foundinconnectivetissueandinternalorgans,butismostprevalentinthedermis,tendons,andbone.Itisa300kDamoleculecomposedoftwoalpha1(I)chainsandonealpha2(I)chainthatspontaneouslyformsatriplehelixscaffoldataneutralpHand37̊C.Thisphenomenoncanbeexploitedtopromotecellattachment,growth,differentiation,migration,andtissuemorphogenesisduringdevelopment.ToprovidethemoststandardizedCollagenIforusein3-Dcultures,aspecialprocessisemployedtoprovidematerialatastandardconcentrationofapproximately5mg/mL.Thismaterialisthenincorporatedina3-Dculturetovalidateefficacy.
Cultrex®3DCultureMatrix®CollagenI isapurifiedstromalECMprotein thathasbeendeveloped,producedandqualifiedspecificallyforusein3Dculturestudies.
Qualitycontrolspecifications:
- Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
- Endotoxinconcentration≤20EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
- NomycoplasmacontaminationdetectedbyPCR.
- Proteinconcentrationof5mg/ml.
- Providedin 20mMAceticAcid,pH3.4–3.6.
- BIOLOGicalactivitydeterminedforeachlot:
- 3-DCulture:CollagenIformsagelwhendilutedto0.4mg/mlatneutralpHandpromotesattachmentandgrowthofmurineendothelialSVEC4-10cells.
- CellAttachment:TestedfortheABIlitytopromotecellattachmentandspreADIngofHT1080humanfibrosarcomacells.
“OrganotypicCultureofUntransformedandTumOrigenicPrimaryMammaryEpithelialCells”
MartinJechlinger
ColdSpringHarbProtoc,May2015;2015:pdb.prot078295
http://cshprotocols.cshlp.org/cgi/content/abstract/2015/5/pdb.prot078295