Description

Cultrex®BasementMembraneExtract(BME)isasolubleformofbasementmembranepurifiedfromEngelbreth-Holm-Swarm(EHS)tumor.Thisextractprovidesanaturalextracellularmatrixhydrogelthatpolymerizesat37°Ctoformareconstitutedbasementmembrane.Basementmembranesarecontinuoussheetsofspecializedextracellularmatrixthatformaninterfacebetweenendothelial,epithelial,muscle,orneuronalcellsandtheiradjacentstromaandthatplayanessentialroleintissueorganizationbyinfluencingcelladhesion,migration,proliferation,anddifferentiation.ThemajorcomponentsofBMEincludelaminin,collagenIV,entactin,andheparansulfateproteoglycans.

Cultrex®3DCultureMatrix®RGFBMEisaBasementMembraneMatrixthathasbeendeveloped,producedandqualifiedspecificallyforusein3Dculturestudies.

Qualitycontrolspecifications:

• Mousecoloniesareroutinelyscreenedforpathogensusing mouseantibodyproduction(MAP)testing.
• Engelbreth-Holm-Swarm(EHS)tumorisLDEV-free.
• PathClear®–testednegativebyPCRfor17bacterialandvirusstrainstypicallyincludedinmouseantibodyproduction(MAP)testing,plus13additionalmurineinfectiousagentsincludingLDEV,foratotalof31organismsandviruses.
• Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
• Endotoxinconcentration≤8EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
• Proteinconcentrationof14–16mg/ml.
• NomycoplasmacontaminationdetectedbyPCR.
• ProvidedinDulbecco’sModifiedEagle’smedium(DMEM)withoutphenolred,with10µg/mlgentamicinsulfate.
• GelstABIlitytestedforaperiodof14daysat37°C.
• BIOLOGicalactivitydeterminedforeachlot:
  • Tubeformationassay–BMEpromotesformationofcapillary-likestructuresbyhuman(HBMVEC;HUVEC)ormouse(SVEC4-10)endothelialcells.
  • 3DCulture:BasementMembraneExtractpromotesdifferentiationofahumanepithelialcelllinederivedfrommammarygland(MCF-10A)orhumanprostate(PC-3)intoacinarstructures.

AMPKReversestheMesenchymalPhenotypeofCancerCellsbyTargetingtheAkt–MDM2–Foxo3aSignalingAxis
Chih-ChienChou,Kuen-HaurLee,I-LuLai,DashengWang,XiaokuiMo,SamuelK.Kulp,CharlesL.Shapiro,andChing-ShihChen
CancerRes.,Sep2014;74:4783–4795.
http://cancerres.aacrjournals.org/cgi/content/abstract/74/17/4783

Silencingβ3IntegrinbyTargetedECO/siRNANanoparticlesInhibitsEMTandMetastasisofTriple-NegativeBreastCancer
JennyG.Parvani,ManeeshD.Gujrati,MargaretA.Mack,WilliamP.Schiemann,andZheng-RongLu
CancerRes.,Jun2015;75:2316–2325.
http://cancerres.aacrjournals.org/cgi/content/abstract/75/11/2316

SurvivalOutcomeandEMTSuppressionMediatedbyaLectinDomainInteractionofEndo180andCD147
MercedesRodriguez-Teja,JulianH.Gronau,AiMinamidate,StevenDarby,LukeGaughan,CraigRobson,FrancescoMauri,JonathanWaxman,andJustinSturge
Mol.CancerRes.,Mar2015;13:538–547.
http://mcr.aacrjournals.org/cgi/content/abstract/13/3/538

“Invitrodifferentiationofhumanamnioticepithelialcellsintoinsulinproducing3Dspheroids”
“BernardOkere,FrancescoAlviano,RobertaCosta,DanielaQuaglino,FrancescaRicci,MassimoDominici,PaoloPaolucci,LauraBonsi,andLorenzoIughetti”
InternationalJournalofImmunopathologyandPharmacology
http://iji.sagepub.com/cgi/content/abstract/28/3/390

Trevigen提供多种产品来测量氧化应激和DNA损伤。我们的彗星实验®产品线是为彗星试验，其中包括试剂，试剂盒，设备和分析软件的唯一完整和优化的商用平台。我们的TACS ®（Trevigen公司凋亡的细胞系统）产品已被广泛使用，并列举多年来，研究人员对他们的无与伦比的品质认可。