Description

4690-096-k_calculation_worksheetv2

ThishomogeneousassaycouplesPARP-1autoribosylationtoafluorescentgeneratingcyclingassay.InhibitorsareidentifiedbyanincreaseinfluorescentsignalwhenPARP-1activityisinhibited.

FEATURES:

• Highlysensitivefluorescentassay
• Detectaslittleas10%inhibitionofPARPActivity
• InhibitorsidentifiedbyincreaseinFluorescentSignal
• Nowashsteps
• 96testsize
• Rapid–Assaytakes~1.5hr

APPLICATIONS:
ForthescreeningofcandidatePARPinhibitorsanddeterminationofrelativeIC50values.

InsightsintoPARPInhibitors’SelectivityUsingFluorescencePolarizationandSurfacePlasmonResonanceBindingAssays
GianlucaPapeo,NillaAvanzi,SerenaBettoni,AntonellaLeone,MauroPaolucci,RitaPerego,FrancescaQuartieri,FedericoRiccardi-Sirtori,SandrineThieffine,AlessiaMontagnoli,andRositaLupi
JBiomolScreen,Jun2014;10.1177/1087057114538319.
http://jbx.sagepub.com/cgi/content/abstract/1087057114538319v1

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