Description

ThisELISAbasedassaydetectsbiotinylatedpoly(ADP-ribose)depositedbyPARP-1ontoimmobilizedhistonesina96-wellformat.TheadditionofStrep-HRP(biotin-bindingprotein)andacolorimetricHRPsubstrateyieldsrelativeabsorbancethatcorrelateswithPARP-1activity.

FEATURES:

• Colorimetricreadout
• 96strip-wellformat
• Sensitive–detects10mUPARP/well
• AssayTime~3hrs

APPLICATIONS:
FortheinvitroscreeningofcandidatePARP-1inhibitorsanddeterminationofIC50values.

EffectofSmall-MoleculeModificationonSingle-CellPharmacokineticsofPARPInhibitors
GregM.Thurber,ThomasReiner,KatherineS.Yang,RainerH.Kohler,andRalphWeissleder
Mol.CancerTher.,Apr2014;13:986–995
http://mct.aacrjournals.org/cgi/content/abstract/13/4/986

CombinatorialEffectsofPARPInhibitorPJ34andHistoneDeacetylaseInhibitorVorinostatonLeukemiaCellLines
EWAJASEK,MARIUSZGAJDA,GRZEGORZJ.LIS,MAŁGORZATAJASIŃSKA,andJANA.LITWIN
AnticancerRes,Apr2014;34:1849–1856.
http://ar.iiarjournals.org/cgi/content/abstract/34/4/1849

Silencingofpoly(ADP-ribose)polymerase-1suppresseshyperstretch-inducedexpressionofinflammatorycytokinesinvitro
Wang,J.,Liu,L.,Xia,Y.andWu,D.
ActaBiochimBiophysSin,May2014;10.1093/abbs/gmu035.
http://abbs.oxfordjournals.org/cgi/content/abstract/gmu035v1

NewInsightsintoPARPInhibitors’EffectonCellCycleandHomology-DirectedDNADamageRepair
PetarJelinicandDouglasA.Levine
Mol.CancerTher.,Jun2014;13:1645–1654.
http://mct.aacrjournals.org/cgi/content/abstract/13/6/1645

DisruptionofWnt/β-CateninSignalingandTelomericShorteningAreInextricableConsequencesofTankyraseInhibitioninHumanCells
“OzlemKulak,HuaChen,BrodyHolohan,XiaofengWu,HuaweiHe,DominikaBorek,Zbyszek
Otwinowski,KiyoshiYamaguchi,LaurenA.Garofalo,ZhiqiangMa,WoodringWright,ChuoChen,JerryW.Shay,XuewuZhang,andLawrenceLum”
Mol.Cell.Biol.,Jul2015;35:2425–2435.
http://mcb.asm.org/cgi/content/abstract/35/14/2425

Acyl-CoA-bindingdomaincontaining3modulatesNAD+metabolismthroughactivatingpoly(ADP-ribose)polymerase1
YongChen,SookheeBang,SoohyunPark,HanyuanShi,andSangwonF.Kim
Biochem.J.,Jul2015;469:189–198.
http://www.biochemj.org/cgi/content/abstract/469/2/189

Trevigen提供多种产品来测量氧化应激和DNA损伤。我们的彗星实验®产品线是为彗星试验，其中包括试剂，试剂盒，设备和分析软件的唯一完整和优化的商用平台。我们的TACS ®（Trevigen公司凋亡的细胞系统）产品已被广泛使用，并列举多年来，研究人员对他们的无与伦比的品质认可。