Description

ThisELISAbasedassaydetectspoly(ADP-ribose)depositedbyTankyrase1ontoimmobilizedhistonesina96-wellformat.Ananti-PARmonoclonaldetectingantibodyfollowedbyadditionofagoatanti-rabbitIgG-HRPsecondaryandacolorimetricHRPsubstrateyieldsrelativeabsorbancethatcorrelateswithTankyrase1activity.

FEATURES:

• Colorimetric,non-rADIoactiveformat
• Higherthroughput
• 96testsize
• Sensitivitydownto0.1mUnitsofTankyrase1.

APPLICATIONS:
ForthescreeningofcandidateTankyrase1inhibitorsanddeterminationofIC50values.

“ThePoly(ADP-ribose)PolymeraseEnzymeTankyraseAntagonizes
Activityoftheβ-CateninDestructionComplexthrough
ADP-ribosylationofAxinandAPC2”
“HeatherE.Croy,CaitlynN.Fuller,JemmaGiannotti,PaigeRobinson,AndrewV.A.Foley,RobertJ.
Yamulla,SeanCosgriff,BradfordD.Greaves,RyanA.vonKleeck,HyunHyungAn,CatherineM.Powers,
JulieK.Tran,AaronM.Tocker,KimberlyD.Jacob,BeckleyK.Davis,andDavidM.Roberts”
J.Biol.Chem.,Jun2016;291:12747–12760.
http://www.jbc.org/cgi/content/abstract/291/24/12747

Trevigen提供多种产品来测量氧化应激和DNA损伤。我们的彗星实验®产品线是为彗星试验，其中包括试剂，试剂盒，设备和分析软件的唯一完整和优化的商用平台。我们的TACS ®（Trevigen公司凋亡的细胞系统）产品已被广泛使用，并列举多年来，研究人员对他们的无与伦比的品质认可。