Description

NOTE: StartingJanuary1st,2017freesamplesizesof Cultrex® OrganoidHarvestingSolution, Cultrex®BMEType2, and Cultrex®BMETypeR1 willbeaddedtoyourorder. Theseproductsarealsosoldindividually,moreinformationonTrevigen®’sCultrexOrganoidproductscanbefoundhere.

Roofplate-specificSpondin-1(R-Spondin1orRspo1),alsoknownasCRISTIN3,isa27kDasecretedactivatorproteinthatbelongstotheR-Spondinfamily.R-SpondinspositivelyregulateWnt/beta-cateninsignaling,mostlikelybyactingasaligandforLGR4-6receptorsandaninhibitorforZNRF3.R-Spondin-1inducesproliferationofintestinalcryptepithelialcells,increasesintestinalepithelialhealing,andsupportsintestinalepithelialstemcellrenewal.

Features:

• RobustandstableexpressionofR-Spondin1.
• Twoepitopetagsprovideflexibilitytopurifyanddetecttheexpressedprotein.
• AccesIBLesourceofeitherpurifiedR-Spondin1orR-Spondin1conditionedmedium.
• Suitabletosupplementbothhumanandmurineorganoidculturemedium.

Qualitycontrolspecifications:

• NegativebyPCRforMycoplasma.
• ExpressiondetectedbyWesternBlot–Abandofapproximately70kDacorrespondingtoHA-R-Spondin1-Fcisdetectedintheconditionedmediumofcellsafter7daysofculture.
• BIOLOGicalactivitydeterminedforeachlot:
  • TopFlashassay–HA-R-Spondin1-FcmustinducetheexpressionofluciferaseunderthecontrolofaTCF/LEFpromoter.

Trevigen提供多种产品来测量氧化应激和DNA损伤。我们的彗星实验®产品线是为彗星试验，其中包括试剂，试剂盒，设备和分析软件的唯一完整和优化的商用平台。我们的TACS ®（Trevigen公司凋亡的细胞系统）产品已被广泛使用，并列举多年来，研究人员对他们的无与伦比的品质认可。