Description
7501-500-k_calculation_worksheet
IntheHTSuperoxideDismutaseAssay,superoxiderADIcalions,generatedfromtheconversionofxanthinetouricacid,andhydrogenperoxidebyxanthineoxidase(XOD),convertWST-1toWST-1-formazan.WST-1-formazanabsorbslightat450nm.SODsreducesuperoxideionconcentrationsandtherebylowertherateofWST-1formazanformation.TheextentofreductionintheappearanceofWST-1formazanisameasureofSODactivitypresentinexperimentalsamples.Thisassayisfreeofinterferencebyothercatalyticactivities,andunlikesomeotherassaykitsforSOD,thissystemisnotgreatlydisturbedbytracemetals.
FEATURES:
- 96wellformatforhighthroughputscreening
- Assayperformedinaslittleas6minutes
- Suitableformammaliantissuesandcelllysates
- Suitablefortheassayof(Mn2+)-SOD,(Fe2+)-SOD,and(Cu/Zn)-SOD
APPLICATIONS:
CalculatingrelativeSODactivitybymeasuringthepercentinhibitionoftherateofformationofWST-1formazan.
| Catalog#7501-500-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 7501-500-01 | SODStandard | 1 |
| 7501-500-03 | XanthineOxidase(XOD) | 1 |
| 7501-500-02 | 10XSODBuffer | 1 |
| 7501-500-04 | 10XXanthineSolution | 1 |
| 7501-500-05 | 20%TritonX-100 | 1 |
| 7501-500-06 | WST-1Reagent | 1 |
| 7512-100-06 | 96-WellPlate | 1 |
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