Description

IntheSuperoxideDismutaseAssay,ionsgeneratedfromtheconversionofxanthinetouricacid,andhydrogenperoxidebyxanthineoxidase(XOD),convertNBTtoNBT-diformazan.NBT-diformazanabsorbslightat550nm.SODsreducesuperoxideionconcentrationsandtherebylowertherateofNBT-diformazanformation.TheextentofreductionintheappearanceofNBT-diformazanisameasureofSODactivitypresentinexperimentalsamples.Theassayisfreeofinterferencebyothercatalyticactivities,andisidealforassayingSODinmammaliancelllysates.Thekitcontainstheproperlysisbufferandthereagentsneededfor100experimentaltests,50positivecontrols,and50negativecontrols.UnlikesomeotherassaykitsforSOD,thissystemisnotgreatlydisturbedbytracemetals.Eachassayrequiresonlyaboutfiveminutes,andafterasimplecalculation,thepercentinhibitionoftheformationofNBT-diformazanbySODisconvertedtotherelativeactivityofthesample.

FEATURES:

• Suitableformammaliancells.
• Eachsampletakesonly5minutes.
• ContainsSODfor50positivecontrols.
• Suitablefortheassayof(Mn²⁺)-SOD,(Fe²⁺)-SOD,and(Cu/Zn)^–SOD.

APPLICATIONS:

CalculatingSODactivitybymeasuringthereductionofNBT-diformazanincellextracts.

RosiglitazoneCausesCardiotoxicityviaPeroxisomeProliferator-ActivatedReceptor{gamma}-IndependentMitochondrialOxidativeStressinMouseHearts
HuameiHe,HaiTao,HuiXiong,ShengZhongDuan,FrancisX.McGowan,Jr.,RichardM.Mortensen,andJamesA.Balschi
Toxicol.Sci.,Apr2014;138:468–481.
http://toxsci.oxfordjournals.org/cgi/content/abstract/138/2/468

Protectiveactivityofanovelresveratrolanalogue,HS-1793,againstDNAdamagein137Cs-irrADIatedCHO-K1cells
Jeong,M.H.,Yang,K.M.,Jeong,D.H.,Lee,C.G.,Oh,S.J.,Jeong,S.K.,Lee,K.W.,Jo,Y.R.andJo,W.S.
J.RadiatRes,May2014;55:464–47
http://jrr.oxfordjournals.org/cgi/content/abstract/55/3/464

Trevigen提供多种产品来测量氧化应激和DNA损伤。我们的彗星实验®产品线是为彗星试验，其中包括试剂，试剂盒，设备和分析软件的唯一完整和优化的商用平台。我们的TACS ®（Trevigen公司凋亡的细胞系统）产品已被广泛使用，并列举多年来，研究人员对他们的无与伦比的品质认可。