Description

7512-100-k_cuvette_assay_calculation_worksheet

7512-100-k_plate_assay_calculation_worksheet

TetramericGlutathionePeroxidasecatalyzesthereductionofH2O2towater,andorganicperoxidestothecorrespondingstablealcohols,byusingglutathioneasasourceofreducingequivalents.Itrequiresseleniumasacofactorandcontainsaselenocysteineaminoacidresidueintheactivesiteofeachmonomerthatparticipatesintheactualmechanismoftheenzyme.GlutathioneReductase(GR),providedwitheachkit,thenreducestheoxidizedglutathionetocompletethecycle.TheoxidationofNADPHtoNADP+isaccompaniedbyadecreaseinabsorbanceat340nm.Therateofdecreaseintheabsorbanceat340nmisdirectlyproportionaltotheGlutathionePeroxidaseactivityinthesample.

FEATURES:

• Suitablefora96wellorcuvetteformat
• Suitableforplasma,erythrocytelysates,tissuehomogenates,andcelllysates

APPLICATIONS:

Calculatingglutathioneperoxidaseactivity.

Glutathione-MediatedDetoxificationofHalobenzoquinoneDrinkingWaterDisinfectionByproductsinT24Cells
JinhuaLi,WeiWang,HongquanZhang,X.ChrisLe,andXing-FangLi
Toxicol.Sci.,Oct2014;141:335–343.
http://toxsci.oxfordjournals.org/cgi/content/abstract/141/2/335

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