Description

4418-096-k_calculation_worksheet

InanELISAassay,immobilizedgamma-H2AXantibodyinthewellsofa96-wellplatecapturesgamma-H2AXfromsamplelysate. IncubationwithaH2AXdetectingantibody,followedbyadditionofagoatanti-mouseHRPconjugateandachemiluminescentHRPsubstrateyieldsrelativelightunits(RLU)thatdirectlycorrelateswiththeamountofgamma-H2AXinthesample.Thegamma-H2AXpolyclonalantibodymaybepurchasedseparately.

FEATURES:

• Chemiluminescent,non-rADIoactiveELISA
• Pre-coated96wellcaptureantibodyplate
• Dynamicrangefrom10pMto800pM
• Sensitivitywith5pMofgamma-H2AX

APPLICATIONS:
Quantitationofgamma-H2AXlevelsinperipheralbloodmononuclearcells,tissueculturecells,andtissuebiopsies.

Abstract2423:DevelopmentofavalidatedhighthroughputELISAassayforgammaH2AXasapharmacodynamicMarker
JayGeorgeandHaiXu
CancerRes.,Oct2014;74:2423.
http://cancerres.aacrjournals.org/cgi/content/abstract/74/19_Supplement/2423}

Engineeredcellulargene-replacementplatformforselectiveandinducIBLeproteolyticprofiling
CharlesW.Morgan,JuanE.Diaz,SamanthaG.Zeitlin,DanielC.Gray,andJamesA.Wells
PNAS,Jul2015;112:8344–8349.
http://www.pnas.org/cgi/content/abstract/112/27/8344

Trevigen提供多种产品来测量氧化应激和DNA损伤。我们的彗星实验®产品线是为彗星试验，其中包括试剂，试剂盒，设备和分析软件的唯一完整和优化的商用平台。我们的TACS ®（Trevigen公司凋亡的细胞系统）产品已被广泛使用，并列举多年来，研究人员对他们的无与伦比的品质认可。