TACS•XL®embodiesamorerobustapproachfortheinsitudetectionofapoptosisthanothermethods.TheTACS•XL®Kits arebasedonincorporationofbromodeoxyuridine(BrdU)atthe3’OHendsoftheDNAfragmentsthatareformedduringapoptosis.TheincorporationofBrdUbyTdTismoreefficientthaneitherbiotinylatedordigoxigeninlabelednucleotidesusedinotherterminaldeoxynucleotidyltransferasedUTPnickendlabeling(TUNEL)-basedassays.Thedetectionsystemutilizesabiotinconjugatedanti-BrdUantibodyandstreptavidin-horserADIshperoxidase.Thecombinationofantibodyspecificitywiththesignalenhancingpropertiesofbiotinstreptavidinresultsinprecisecellularlabelingandthehighestsignal-to-noiseratioobservedincompetitivetesting.
TheTACS•XL®BasicKitprovidesthereagentsnecessaryforroutinelabeling,includingtwopermeABIlizationreagents,labelingbuffersandreagents,Strep-HRPandTACS-Nuclease™reagentsforgeneratingpositivecontrolswithyourownsamples.It isidealforresearcherswhoselabsareequippedforstandardimmunohistochemicalproceduresinvolvinghorseradish-peroxidasewanttoadapttheassayforfluorescencedetection. Thekitdoesn’tnotcontainanyperoxydase-substrateorcounterstain. Add-onTACS•XL® BlueLabelModuleand TACS•XL® DABModule detectionmodules areavailableseparately.
Pleaserefertoourcomponentslistingfordetails.
Catalog#4828-30-Kincludes: | ||
CatalogNumber | Description | Qty |
4800-30-01 | ProteinaseK | 1 |
4800-30-06 | Streptavidin-HRP | 1 |
4810-30-02 | TACS®2TdTLabelingBuffer | 1 |
4810-30-03 | TACS®2TdTStopBuffer | 1 |
4810-30-05 | TdTEnzyme | 1 |
4828-30-04 | B-dNTPMix | 1 |
4828-30-06 | anti-BrdUantibody | 1 |
4828-30-12 | Strep-Diluent | 1 |
4876-05-01 | Cytonin™ | 1 |
Trevigen提供多种产品来测量氧化应激和DNA损伤。我们的彗星实验®产品线是为彗星试验,其中包括试剂,试剂盒,设备和分析软件的唯一完整和优化的商用平台。我们的TACS ®(Trevigen公司凋亡的细胞系统)产品已被广泛使用,并列举多年来,研究人员对他们的无与伦比的品质认可。