Description

ThehighcellularityofcardiactissuepresentsproblemsinpermeABIlization,sotheCardioTACS™Kitcomeswithtwopermeabilizationreagentstoprovideoptions.ThekitisbasedonDNAend-labelingusingterminaldeoxynucleotidyltransferase(TdT)andamodifiednucleotidethatissubsequentlydetectedusingourTACSBlueLabel™detectionsystem.TrevigenhasdevelopedanexclusiveapoptosisgradeTdTenzymethatwhencoupledwiththeTACSBlueLabel™solution,provideslabeling20to50timesmoresensitivethanstandarddiaminobenzidine(DAB)labelingmethods.Toensureeaseofdatainterpretationwhenthenumbersofapoptoticcellsarelow,CardioTACS™includesNuclearFastRedCounterstainwhichprovidessuperbcontrasttotheTACSBlueLabel™inapoptoticcells.

Inaddition,thekitincludesTACS-Nuclease™togenerateapositivecontrolinyourownsample.Thepositivecontrolprovidesaninternalcontrolforpermeabilizationandlabelingsoadditionaloptimizationisnominalandthedatacanbeinterpretedwithconfidence.Thiscompletekitprovidesallthereagentsrequiredforlabelingincludingtwopermeabilizationreagents,labelinganddetectionreagents,stopbuffer,counterstain,TACS-Nuclease™reagent,andaprotocoldetailingincubationtimesandreagentconcentrations.

FEATURES:

• Performancetestedonheart-derivedsamples.
• IncludesbothProteinaseKandexclusiveCytonin™non-enzymaticpermeabilizationreagents.
• IncludesTACS-Nuclease™solutionforpreparingsample-dependentpositivecontrols.
• Helpsresolveuniqueproblemsencounteredwhendetectingapoptoticcardiaccells.

APPLICATIONS:

• Insitudetectionofapoptosisinfixedfrozen,paraffinembedded,orplasticembeddedcardiaccellsandtissues.
• Assistsintheidentificationofapoptoticmorphologies.

TheInfluenceofAgeonSerumConcentrationsofCardiacTroponinI:ResultsinRats,Monkeys,andCommercialSera
EugeneH.Herman,AlanKnapton,YongminLiu,StevenE.Lipshultz,JoelEstis,JohnTodd,RuthA.Woodward,ThomasCochran,JunZhang,andMiriamC.Poirier
ToxicolPathol,Jul2014;42:888–896.
http://tpx.sagepub.com/cgi/content/abstract/42/5/888

“GSK-3βheterozygousknockoutiscardioprotectiveinaknockinmouse
modeloffamilialdilatedcardiomyopathy”
“RashaM.S.M.Mohamed,SachioMorimoto,IslamA.A.E.-H.Ibrahim,Dong-YunZhan,Cheng-KunDu,MasakiArioka,TatsuyaYoshihara,FumiTakahashi-Yanaga,andToshiyukiSasaguri”
AmJPhysiolHeartCircPhysiol,Jun2016;310:H1808–H1815
http://ajpheart.physiology.org/cgi/content/abstract/310/11/H1808

“Phosphodiesterase5InhibitionLimitsDoxorubicin-inducedHeartFailurebyAttenuatingProteinKinaseGIαOxidation”
“OleksandraPrysyazhna,JosephRobertBurgoyne,JennaScotcher,StevenGrover,DavidKass,andPhilipEaton”
J.Biol.Chem.,Aug2016;291:17427–17436.
http://www.jbc.org/cgi/content/abstract/291/33/17427

Trevigen提供多种产品来测量氧化应激和DNA损伤。我们的彗星实验®产品线是为彗星试验，其中包括试剂，试剂盒，设备和分析软件的唯一完整和优化的商用平台。我们的TACS ®（Trevigen公司凋亡的细胞系统）产品已被广泛使用，并列举多年来，研究人员对他们的无与伦比的品质认可。