Description

Thekithasbeendevelopedtoovercomethecommondifficultiesuniquetoneuronalsamplesincludingthefragilenatureofbraintissuesections,highbackgroundproblems,poorcounterstainingwithcommondyes,andtheneedtoperformduallabelingexperimentstodetectcellspecificantigensinconjunctionwithapoptoticcells.AkeyfeatureisNeuroPore™,aproprietarypermeABIlizationreagentthatgentlypermeabilizessampleswhileretainingcellmorphology.NeuroPore™alsocontainsblockingreagentstoallowitsuseasanantibodydiluentinimmunohistochemistryandtoreducebackgroundstaining.

DNAfragmentsgeneratedbyapoptosisareend-labeledwithmodifiednucleotidesusingahighlypurifiedterminaldeoxynucleotidyltransferaseenzyme(TdT).TheincorporatednucleotidesaredetectedusingahorserADIshperoxidasesystemthatcatalyzestheconversionofdiaminobenzidine(DAB)intoavisIBLedarkbrownprecipitate.NeuroTACS™IIcontainstheBlueCounterstaintoallowvisualizationofallcellswithinthesamplewithgoodcontrasttothebrownDABprecipitate.ADABenhancerisalsoprovidedwiththekitfortheoptiontointensifyordarkenDABstaining.Inaddition,theBlueCounterstainiscompatiblewiththered-coloredsubstratesusedforphosphatasedetectionindoublelabelingexperiments.Theprotocolincludesdetailsforlabelinginsituforapoptosisandantigendetectiononthesamesample.Toensurethatyourownsampleshavebeenprocessedandpermeabilizedcorrectly,weprovideTACS-Nuclease™,auniquereagentusedtogenerateapositivecontrolwithyourownsamples.Thisprovidesahighdegreeofconfidencefordatainterpretationandcanhelppinpointproblemstepsinthelabelingprocedure.

FEATURES:

• Uniquebuffersystemproducesmoreconsistentlabeling.
• Performancetestedonbrainsections.
• IncludesexclusiveNeuroPore™permeabilizationreagent.
• IncludesTACS-Nuclease™solutionforpreparingsample-dependentpositivecontrols.
• Helpsresolveuniqueproblemsencounteredwhendetectingapoptoticneuronalcells.

APPLICATIONS:

• Insitudetectionofapoptosisinfixedfrozen,paraffinembedded,orplasticembeddedcellsandtissues.
• Assistsintheidentificationofapoptoticmorphologies.

DifferencesinthePhagocyticResponseofMicrogliaandPeripheralMacrophagesafterSpinalCordInjuryandItsEffectsonCellDeath,
AndrewD.GreenhalghandSamuelDavid
J.Neurosci.,Apr2014;34:6316–6322.
http://www.jneurosci.org/cgi/content/abstract/34/18/6316

HypoxiaInducibleFactor-1{alpha}(HIF-1{alpha})IsRequiredforNeuralStemCellMaintenanceandVascularStabilityintheAdultMouseSVZ
LuLi,KateM.Candelario,KelseyThomas,RuthWang,KandisWright,AmberMessier,andLeeAnnaCunningham

SynapticSynthesis,Dephosphorylation,andDegradation:ANOVELPARADIGMFORANACTIVITY-DEPENDENTNEURONALCONTROLOFCDKL5
PaoloLaMontanara,LauraRusconi,AlbinaLocarno,LiaForti,IsabellaBarbiero,MarcoTramarin,ChetanChandola,CharlotteKilstrup-Nielsen,andNicolettaLandsberger
J.Biol.Chem.,Feb2015;290:4512–4527.
http://www.jbc.org/cgi/content/abstract/290/7/4512

Trevigen提供多种产品来测量氧化应激和DNA损伤。我们的彗星实验®产品线是为彗星试验，其中包括试剂，试剂盒，设备和分析软件的唯一完整和优化的商用平台。我们的TACS ®（Trevigen公司凋亡的细胞系统）产品已被广泛使用，并列举多年来，研究人员对他们的无与伦比的品质认可。