Description

Intheintrinsicapoptosispathway,mitochondrialpermeABIlitytransitionisanimportanteventwhereintheelectRochemicalgrADIent(referredtoasdelta-psiorΔΨm)acrossthemitochondrialmembranecollapses.Thiscollapseoccursthroughtheformationofchannelsorporesintheoutermitochondrialmembrane,whichinvolvesBaxinsertionandoligomerization,followedbythereleaseofCytochromeCintothecytoplasm.

TheDePsipher™Kitusesauniquecationicdye(5,5’6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimidazolyl-carbocyanineiodide)toindicatethelossofΔΨm.Thedyereadilyenterscellsandfluorescesbrightlyredinitsmultimericformwithinhealthymitochondria.Inapoptoticcells,themitochondrialmembranepotentialcollapses,andtheDePsipher™reagentcannotaccumulatewithinthemitochondria.Inthesecells,DePsipher™returnstoitsgreenfluorescentmonomericform.Apoptoticcells,showingprimarilygreenfluorescence,arethuseasilydifferentiatedfromhealthycellswhichshowredfluorescence.Theaggregateredformhasabsorption/emissionmaximaof585/590nm,andthegreenmonomericformhasabsorption/emissionmaximaof510/527nm.Bothapoptoticandhealthycellscanbevisualizedsimultaneouslybyepifluorescencemicroscopyusingawideband-passfilter.

TheDePsipher™reagentiseasytouse.SimplyresUSPendthereagentinreactionbufferorculturemedia(withorwithoutthestabilizersolution),addtoyourcells,incubatefor15to20minutes,washandanalyzebyflowcytometryormicroscopy.Visualizationbymicroscopyallowsarapidinspectionandqualificationofapoptosis.Flowcytometricanalysisallowseasyquantitationofcelldeathasevidencedbymitochondrialpotentialbreakdown.

FEATURES:

• Simple.JustaddDePsipher™reagenttomediaorreactionbuffer.
• UniqueStabilizerSolutionimprovesresults.
• Fast.Takesonly20minutes.
• FlexIBLe.Viewcellsbyepifluorescenceorconfocalmicroscopy,oranalyzecellsbyflowcytometry.

APPLICATIONS:

• Flowcytometry
• Epifluorescencemicroscopy
• Confocalmicroscopy

Trevigen提供多种产品来测量氧化应激和DNA损伤。我们的彗星实验®产品线是为彗星试验，其中包括试剂，试剂盒，设备和分析软件的唯一完整和优化的商用平台。我们的TACS ®（Trevigen公司凋亡的细胞系统）产品已被广泛使用，并列举多年来，研究人员对他们的无与伦比的品质认可。