Description

Cultrex®BasementMembraneExtract(BME)isasolubleformofbasementmembranepurifiedfromEngelbreth-Holm-Swarm(EHS)tumor.Thisextractprovidesanaturalextracellularmatrixhydrogelthatpolymerizesat37°Ctoformareconstitutedbasementmembrane.Basementmembranesarecontinuoussheetsofspecializedextracellularmatrixthatformaninterfacebetweenendothelial,epithelial,muscle,orneuronalcellsandtheiradjacentstromaandthatplayanessentialroleintissueorganizationbyinfluencingcelladhesion,migration,proliferation,anddifferentiation.ThemajorcomponentsofBMEincludelaminin,collagenIV,entactin,andheparansulfateproteoglycans.

Cultrex®BMEType2isaBasementMembraneMatrixthathasbeendeveloped,producedandqualifiedspecificallyforrobustorganoidcultures.

Qualitycontrolspecifications:

• Mousecoloniesareroutinelyscreenedforpathogensusing mouseantibodyproduction(MAP)testing.
• Engelbreth-Holm-Swarm(EHS)tumorisLDEV-free.
• PathClear®–testednegativebyPCRfor17bacterialandvirusstrainstypicallyincludedinmouseantibodyproduction(MAP)testing,plus13additionalmurineinfectiousagentsincludingLDEV,foratotalof31organismsandviruses.
• Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
• Endotoxinconcentration≤8EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
• Proteinconcentrationof12–18mg/ml.
• NomycoplasmacontaminationdetectedbyPCR.
• ProvidedinDulbecco’sModifiedEagle’smedium(DMEM)withoutphenolred,with10µg/mlgentamicinsulfate.
• GelstABIlitytestedforaperiodof14daysat37°C.
• BIOLOGicalactivitydeterminedforeachlot:
  • Tubeformationassay–BMEpromotesformationofcapillary-likestructuresbyhuman(HBMVEC;HUVEC)ormouse(SVEC4-10)endothelialcells.
  • Organoidculture:BasementMembraneExtractpromotesgrowthandexpansionofmousesmallintestineorganoidProgenitorcells.

“BasementMembrane-BasedGlucoseSensorCoatingsEnhance
ContinuousGlucoseMonitoringinVivo”
UlrikeKlueh,YiQiao,CarolineCzajkowski,IzabelaLudzinska,OmarAntar,andDonaldL.Kreutzer
JournalofDiabetesScienceandTechnology,Sep2015;9:957–965.
http://dst.sagepub.com/cgi/content/abstract/9/5/957

Trevigen提供多种产品来测量氧化应激和DNA损伤。我们的彗星实验®产品线是为彗星试验，其中包括试剂，试剂盒，设备和分析软件的唯一完整和优化的商用平台。我们的TACS ®（Trevigen公司凋亡的细胞系统）产品已被广泛使用，并列举多年来，研究人员对他们的无与伦比的品质认可。