Description

ThisELISAbasedassaydetectspoly(ADP-ribose)depositedbyPARP-1ontoimmobilizedhistonesina96-wellformat.Ananti-PARmonoclonaldetectingantibodyfollowedbyadditionofagoatanti-rabbitIgG-HRPsecondaryandacolorimetricHRPsubstrateyieldsrelativeabsorbancethatcorrelateswithPARP-1activity. DuringapoptosisPARP-1iscleavedwitharesultingdropinactivity.Etoposideisincludedasacontrolapoptosisinducer.

FEATURES:

• Colorimetricreadout
• 96wellformat
• Highlysensitive–detects0.1mUPARP~500cells
• Dynamicrangebetween0.1to10mUPARP
• Requires10-100ngextractfordetection
• AssayTime~3hrs

APPLICATIONS:

• MeasurePARP-1activityincellextractsbeforeandafterapoptosis.
• ForthescreeningofcandidatePARP-1inhibitorsanddeterminationofIC50values.

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Trevigen提供多种产品来测量氧化应激和DNA损伤。我们的彗星实验®产品线是为彗星试验，其中包括试剂，试剂盒，设备和分析软件的唯一完整和优化的商用平台。我们的TACS ®（Trevigen公司凋亡的细胞系统）产品已被广泛使用，并列举多年来，研究人员对他们的无与伦比的品质认可。